Everything on ice. Why?
Everything on ice. Why?
PUC18 plasmids.
What is a plasmid?
What is special about these plasmids?
Lux plasmids.
What is special about these plasmids?
Adding competent bacteria.
What does "competent bacteria" mean? How did they become competent?
What was already in the eppindorf tube?
Why wear gloves?
Warming the bacteria.
Assume that nutrient broth has already been added to the tubes. What
takes place in the tubes left here during the 30 min?
Fresh agar plate.
What was added to the agar when the agar was made?
Why use that kind of agar?
Adding recombinant E. coli to the agar plate.
Why is the lid only opened partially?
Why must these be sterile?
Streaking the plate.
What does this accomplish?
Why clean up the tables when you are finished?
Bacterial colonies two days later..
Which bacteria did you use?
How did each colony start?
Since these are growing, what does that mean about the success of your lab work?
Which plate(s) could this be?
The control plate two days later.
Same agar. Same bacteria but with no added plasmids.
Why is there no growth of bacterial colonies?
A plate in the dark room.
Which plate(s) could this be? Explain.
Photos by Steve Muzos © Austin Community College
Last updated by Steve Muzos September 13, 2002
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