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PATERNITY TESTING

PARTS 2 & 3

Learning how to use the equipment


   A micropipetter.
 

  Adding a disposable tip.

How often is it necessary to add a new tip?
Why wear gloves?
 

  What do these number mean?

What is the yellow button for?
What is the difference between you use the first stop and the second stop?
 

   What does this fuzzy setting indicate. It is from the instrument shown above.
 

  An empty gel in liquid.

What are the 8 rectangles called?
Do they go all the way through the gel?
 

   Loading solution.

This blue solution has glycerol in it .... why?
What is the name of the container that has the solution in it here?
 

   Filling well #5 with loading solution.

Note that well #2 has some spillage.
 

   All six wells are loaded!
 
 

PATERNITY TESTING

PARTS 4-6

Running an agarose gel


   A gel which is ready to use.

What is the blue thing?   What is its function?
 

  What is in these tubes labeled A, B, C, D, and E?
 

  Electrophoresis apparatus still empty.
 

  How is this used?
 

  Loading lane 5.

What is in the wells?

Why is it blue?
 

   Running the gel
 

  Notice the black wire?

Why are the wells at that end of the apparatus?
 
 

  Power supply running properly.
 
 

   Bubbles from one electrode.
 

  The gel shortly after turning on the power supply.

What is moving out of the wells?

Why is it moving?   Why is it moving that direction?
 

    A few minutes later.

What is in the blue bands?  Be careful!
 
 

  The gel now out of the apparatus.
Why cut off this corner?

Notice where the bands are?   What does that indicate?
 

  The stain supply.
 

  Starting to stain the gel.
 

  The light box.
 

  A result.

Be able to interpret this.

What is in lane 1?

   Saving the gel in water.

Where will this be kept?

Why keep it in water? There are at least two reasons.
 
 
 


Photos by Steve Muzos © Austin Community College

Last updated by Steve Muzos September 13, 2002

<<<<<< Back to Lab Photos page

         <<<< Back to Dr. Muzos' home page                                                                                              <<<< Back to Dr. Muzos'  BIOL 1408 page