Welcome to Microbugz
Introduction
Fundamental Skills
Aseptic Technique
Streak Plate Method of Isolation
Microbial Growth
Ubiquity of Microorganisms
Cultural Characteristics
Growth Patterns in Broth
Fluid Thioglycollate Broth
Anaerobic Jar
Candle Jar
Microscopy and Staining
Intro to Light Microscope
Eukaryotic Microbes
Simple Stains
Negative Stain
Gram Stain
Acid-Fast Stain
Capsule Stain
Endospore Stain
Wet Mount and Hanging Drop
Flagella Stain
Morphological Unknown
Selective Media
6.5% Salt Tolerance Test
Mannitol Salt Agar
Phenylethyl Alcohol Agar
Eosin Methylene Blue Agar
Hektoen Enteric Agar
MacConkey Agar
Differential Tests
Phenol Red Broth
Casease Test
Gelatinase Test
Lipase Test
Starch Hydrolysis
Methyl Red & Vogues-Proskauer Test
Citrate Test
SIM Medium
Nitrate Reduction Test
Urease Test
Triple Sugar Iron Agar
Decarboxylation Test
DNase Test
Phenylalanine Deaminase Test
Blood Agar
Coagulase Test
Motility Test
Catalase Test
Oxidase Test
Bile Esculin Test
Bacitracin & Optochin Susceptibility
API 20 E Identification System
Mixed Unknown
Medical Microbiology
Antimicrobial Susceptibility Test
Disinfectants
Microbial Genetics
UV Radiation Damage and Repair
Hematology and Serology
Slide Agglutination
Eukaryotic Microbes
Fungi
Protozoans
Helminths
Hookworms
Coccidia
Cryptosporidium parvum (iodine stain)
Characteristics
Parasite will not take up iodine
Uses a traunt stain - red to pink
Mostly found in daycares with small children
Size
Very small
Pathogenic
YES
Cryptosporidium parvum (flourescent stain)