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6.5% Salt Tolerance Test
The salt tolerance test is performed using Tryptic Soy Broth with added sodium chloride (regular table salt) to create an overall salt concentration of 6.5%. It is a selective medium which tests the ability of an organism to survive in a salt-rich environment. Most organisms cannot survive in such an environment. Staphylococci, Enterococci,and Aerococci are expected to grow in this broth; other organisms are not.
You can find more discussion and the testing protocol here.
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The tube above was inoculated with Staphylococcus aureus. Notice the turbidity of the medium; the organism has grown in the salt broth. |
The tube above was inoculated with Streptococcus pyogenes. Notice the clarity of the broth; the organism did not grow in the salt broth. |